Dipolar Order Parameters in Large Systems With Fast Spinning

Dipolar Order Parameters in Large Systems With Fast Spinning

Blog Article

Order parameters are a useful tool for quantifying Vases amplitudes of molecular motions.Here we measure dipolar order parameters by recoupling heteronuclear dipole-dipole couplings under fast spinning.We apply symmetry based recoupling methods to samples spinning under magic angle at 60 kHz by employing a variable flip angle compound inversion pulse.We PERFUME validate the methods by measuring site-specific 15N-1H order parameters of a microcrystalline protein over a small temperature range and the same protein in a large, precipitated complex with antibody.The measurements of the order parameters in the complex are consistent with the observed protein undergoing overall motion within the assembly.